In 2017, the total global shrimp production exceeded 4.3 million tons, a year-on-year increase of about 7.5%, of which China, India, Ecuador, Vietnam, Thailand, Indonesia and other six major producing countries produced more than 80% of global shrimp production. More than 80% of China ’s cultured shrimp production comes from P. vannamei. So far, P. vannamei has developed in China for more than 20 years and has formed a huge industrial chain. However, in recent years, diseases of P. vannamei breeding have emerged endlessly. According to survey data from the Global Aquaculture Alliance (Figure 1), disease and aquaculture costs are the main difficulties and challenges currently facing global South American aquaculture. How to improve the survival rate and reduce the cost of culture of P. vannamei is the necessary way to increase the international competitiveness of P. vannamei culture in China!
With the help of the internationally advanced bio-fermentation technology, Xijie Group's biochemical division has uniquely developed and produced a single Bacillus aerobic fermentation protein powder ?? Soytide in China and even globally. Fast-acting peptide is a product of aerobic solid fermentation of soybean meal from Bacillus. Bacillus grows fast and has strong ability to secrete proteases. It is the preferred strain for fermentation of soybean meal. High digestion and absorption rate, good water stability (Figure 3). Quick-acting peptides contain 107 high-temperature resistant Bacillus, and there are still live bacteria after granulation at high temperature (even after puffing) (Figure 4). Aquatic animals can improve their intestinal health, enhance immunity, improve water quality, and reduce shrimp mortality after eating. . Through the practice in P. vannamei farming, we found that fast-acting peptides can replace fishmeal in shrimp culture and reduce the cost of farming; it has significant effects in enhancing the intestinal health of shrimp, reducing mortality, and improving water quality, and it is worth trying!
Feasibility Study on Fast Food Peptide Replacement of Fish Meal
1, experimental design
A total of 480 healthy P. vannamei juveniles were selected, with an initial weight of 0.5 g. Each diet was processed in 3 replicates, each with 40 shrimps. The dietary treatment of juvenile shrimp consisted of a control group (fishmeal group) and three test groups (quick peptide, quick peptide (sterilized), and fermented soybean meal D, respectively replacing part of the fish meal group). The diet formula is shown in Table 1. The experiment used full feeding, fed 4 times a day for 8 weeks. The test environment is 27.0 ± 1 ℃, equipped with a seawater circulation device (salinity 33ppt).
Table 1. Test diet composition
2, test results
2.1 Effect of fast-acting peptide on growth performance of juvenile shrimp
Regardless of whether it was sterilized or not, the fast-acting peptides successfully replaced the 9-point fish meal. The non-sterile fast-acting peptide group significantly increased the weight gain and specific growth rate of P. vannamei compared with other groups (P <0.05). The sterilized fast peptide still improved the performance of P. vannamei to some extent. The results show that the improvement of the production performance of P. vannamei by the fast-acting peptide benefits from the probiotics and the degradation of the product antigen.
2.2 Effect of fast-acting peptide on non-specific immunity of juvenile shrimp
超 There was no significant difference in the inhibitory rate of superoxide dismutase between the groups (P> 0.05), but the lysozyme activity of juvenile shrimp in the fast-acting peptide group was significantly higher than that in the fishmeal and fermented soybean meal D groups (P <0.05). Among them, the lysozyme activity of the fast-acting peptide (sterilized) group was significantly higher than that of the fermented soybean meal group D, and at the same time, it was also improved to a certain extent compared with the fishmeal group (Figure 6).
Study on the application of salamander peptides to the survival rate and water quality improvement of Penaeus vannamei
1, experimental design
Select 120 healthy P. vannamei shrimps with an initial weight of 4.08 + 0.12g, 3 replicates per group, and 10 young shrimps each. The diet was treated as control group, control & probiotic group (control group (+)), fish meal replacement group (quick peptide group), fish meal replacement & probiotic group (quick peptide (+)), of which probiotics were derived from A commercial brand B (Table 2). The experiment uses full feeding, feeding 4 times a day for 4 weeks. The test environment is 27.0 ± 2 ℃, equipped with seawater circulation device (salinity 33ppt).
Table 2. Experimental design
"X" means not added; "O" means added
2, test results
2.1 Effect of fast-acting peptides on the survival rate of juvenile shrimp
In the 4th week of the experiment, the replacement of test water was stopped, and the mortality of P. vannamei increased in each group, among which the mortality rate of the control group was the highest, as high as 50%, which was significantly higher than the other three groups (P <0.05). However, there was no significant difference in survival rates of the fast-acting peptide group, the fast-acting peptide (+), and the control group (+). The survival rate of the fast-acting peptide group was higher than 60% and slightly higher than that of the control group (+). Survival rate (Figure 5).
2.2 Water-adjusting effect of quick-acting peptides
One week later, the NO2 concentration in the water of the fast-acting peptide group and the fast-acting peptide (+) group was significantly lower than that of the control group (P <0.05). At the same time, the control of NO2 concentration in water by the Bacillus in the fast-acting peptide group was better than that used in this test A commercial brand B. The number of Bacillus per milliliter of water in the fast-acting peptide group and the fast-acting peptide (+) group at the second and fourth weeks of the experiment was significantly higher than that of the control group (P <0.05).
1. The improvement of the production performance of P. vannamei by fast-acting peptides is due to the probiotics and antigen degradation in the product.
2. Fast-acting peptides improve the water quality environment of P. vannamei breeding, enhance immunity, and increase the survival rate of P. vannamei.